In order to analyze ecological values of a regional ecosystem, this paper provided a systematic analysis on the value of regional ecosystem services based on the concept of sustainable development in ecological economics. We have not only estimated the natural capital but also the economic capital and social capital in a regional ecosystem. The total capital of 23 ecosystems serves for 9 sub-ecosystems. There are several main appraisal approaches that could be used in ecosystem valuation. Because the regional ecosystem services are uncertain, this must be considered a minimum estimate. For the total capital, natural capital contributes to more than 1/3 of human welfare in the regional ecosystem every year, and therefore represents part of the total economic and social value. Sustainable development can only be achieved when natural capital is increased in a regional ecosystem.